anti mouse cd3 allophycocyanin apc cy7  (Biogems International)

Journal: Bioorganic & medicinal chemistry

Article Title: Selective photodepletion of malignant T cells in extracorporeal photopheresis with selenorhodamine photosensitizers

doi: 10.1016/j.bmc.2016.05.071

Figure Lengend Snippet: Non-stimulated PBMCs were combined 5:1 with HUT-78 cells. Samples were then photodepleted (PD) with 7.5 × 10−8 M selenorhodamine 5–10 and 5 J cm−2 light. Cell survival was measured 18 h after light exposure and compared to control (non-PD samples) by FACS analysis. The bar graphs represent percent survival of malignant HUT-78, the CD3+ population, and the CD4+ and CD8+ subsets of CD3+ cells after photodepletion with selenorhodamines (a) 5 and 6 and (b) 7–10. The bars represent the mean of three replicate experiments. Error bars are the SEM.

Article Snippet: The following monoclonal antibodies were used and purchased from eBioscience: mouse anti-human α-CD3-Cyanin-7-allophycocyanin (APC-Cy7; clone OKT3); α-CD4-Pacific Blue (PB; clone RPA-T4); α-CD8-fluorescein isothiocyanate (FITC; clone SK1); and α-CD25-Cyanin-7-phycoerytherin (PE-Cy7; clone BC96).

Techniques:

Journal: Bioorganic & medicinal chemistry

Article Title: Selective photodepletion of malignant T cells in extracorporeal photopheresis with selenorhodamine photosensitizers

doi: 10.1016/j.bmc.2016.05.071

Figure Lengend Snippet: Survival of HUT-78 cells and non-stimulated PBMCs following photodepletion with 7.5 × 10 −8 M photosensitizer and 5 J of 600-nm LED light a

Article Snippet: The following monoclonal antibodies were used and purchased from eBioscience: mouse anti-human α-CD3-Cyanin-7-allophycocyanin (APC-Cy7; clone OKT3); α-CD4-Pacific Blue (PB; clone RPA-T4); α-CD8-fluorescein isothiocyanate (FITC; clone SK1); and α-CD25-Cyanin-7-phycoerytherin (PE-Cy7; clone BC96).

Techniques:

Journal: Bioorganic & medicinal chemistry

Article Title: Selective photodepletion of malignant T cells in extracorporeal photopheresis with selenorhodamine photosensitizers

doi: 10.1016/j.bmc.2016.05.071

Figure Lengend Snippet: Survival of HUT-78 cells and SEB-stimulated PBMCs following photodepletion with 7.5 × 10 −8 M selenorhodamine and 5 J of 600-nm LED light a

Article Snippet: The following monoclonal antibodies were used and purchased from eBioscience: mouse anti-human α-CD3-Cyanin-7-allophycocyanin (APC-Cy7; clone OKT3); α-CD4-Pacific Blue (PB; clone RPA-T4); α-CD8-fluorescein isothiocyanate (FITC; clone SK1); and α-CD25-Cyanin-7-phycoerytherin (PE-Cy7; clone BC96).

Techniques:

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Targeting T cell Bioenergetics by Modulating P-glycoprotein Selectively Depletes Alloreactive T cells to Prevent GVHD 1

doi: 10.4049/jimmunol.1402445

Figure Lengend Snippet: PBMCs were stimulated with SEB for 72 hours. (A) Activated (CD3+CD25+) and resting (CD3+CD25−) T cells were then identified by FACS analysis, as shown for one representative experiment. Activated and resting T cells were then isolated, and bioenergetics were measured by real-time extracellular flux analysis. The (B) oxygen consumption rate (OCR) and (C) spare respiratory capacity (SRC) were measured in a basal state, and after the addition of oligomycin (to block ATP synthesis), FCCP (to uncouple ATP synthesis from the electron transport chain), and rotenone (to block complex I of the electron transport chain), as shown for one representative experiment. (D) The mean fluorescent intensity (MFI) of MitoTracker Green, (E) the basal OCR, (F) the basal extracellular acidification rate (ECAR), and (G) the OCR/ECAR ratio are shown for both activated and resting cells. Mean ± SE are plotted from 5 independent experiments. * p < 0.05, ** p < 0.01.

Article Snippet: Reagents for flow cytometry The following monoclonal antibodies were used and purchased from eBioscience: mouse anti-human α-CD3-Cyanin-7-allophycocyanin (APC-Cy7; clone OKT3); α-CD4-Pacific Blue (PB; clone RPA-T4); α-CD8-fluorescein isothiocyanate (FITC; clone SK1); α-CD25-Cyanin-7-phycoerytherin (PE-Cy7; clone BC96); and rat anti-mouse α-CD19-PE (clone 1D3); α-CD3-FITC (clone 17AD); α-CD90.1- Peridinin-chlorophyll protein-Cy5.5 (PerCP-Cy5.5) or –FITC (clone HIS51); α-CD8-PB or –PE or –V500 (clone 53-6.7); α-CD27-PE-Cy7 (clone LG.7F9); α-CD44-FITC or –PerCP-Cy5.5 (clone IM7); α-CD62L-APC-Cy7 (clone MEL-14); α-CD127-FITC (clone A7R34); α-KLRG1 (clone 2F1); INF-γ-FITC (clone XMG1.2); MIP-1α-PE (clone DNT3CC); TNF-α-PE-Cy7 (cloneMP6-XT22); and IL-2-APC (clone JES6-5H4) .

Techniques: Isolation, Blocking Assay

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Targeting T cell Bioenergetics by Modulating P-glycoprotein Selectively Depletes Alloreactive T cells to Prevent GVHD 1

doi: 10.4049/jimmunol.1402445

Figure Lengend Snippet: Stimulated T cells by were identified by FACS analysis for CD3+ and CD25+ coexpression. Resting T cells were identified as CD3+ without expression of CD25. A) A representative histogram of photosensitizer fluorescence is shown. B) Bar graphs represent the ratio of the mean fluorescent intensity (MFI) of CD25+/CD25− T cells for the strong and weak P-gp stimulating photosensitizers. Mean ± SE are plotted from 3 independent experiments. ** p < 0.01.

Article Snippet: Reagents for flow cytometry The following monoclonal antibodies were used and purchased from eBioscience: mouse anti-human α-CD3-Cyanin-7-allophycocyanin (APC-Cy7; clone OKT3); α-CD4-Pacific Blue (PB; clone RPA-T4); α-CD8-fluorescein isothiocyanate (FITC; clone SK1); α-CD25-Cyanin-7-phycoerytherin (PE-Cy7; clone BC96); and rat anti-mouse α-CD19-PE (clone 1D3); α-CD3-FITC (clone 17AD); α-CD90.1- Peridinin-chlorophyll protein-Cy5.5 (PerCP-Cy5.5) or –FITC (clone HIS51); α-CD8-PB or –PE or –V500 (clone 53-6.7); α-CD27-PE-Cy7 (clone LG.7F9); α-CD44-FITC or –PerCP-Cy5.5 (clone IM7); α-CD62L-APC-Cy7 (clone MEL-14); α-CD127-FITC (clone A7R34); α-KLRG1 (clone 2F1); INF-γ-FITC (clone XMG1.2); MIP-1α-PE (clone DNT3CC); TNF-α-PE-Cy7 (cloneMP6-XT22); and IL-2-APC (clone JES6-5H4) .

Techniques: Expressing, Fluorescence